diff --git a/src/bun.js/bindings/ZigGlobalObject.cpp b/src/bun.js/bindings/ZigGlobalObject.cpp
index 74db88cf1f..bfc537aebe 100644
--- a/src/bun.js/bindings/ZigGlobalObject.cpp
+++ b/src/bun.js/bindings/ZigGlobalObject.cpp
@@ -293,6 +293,14 @@ extern "C" void JSCInitialize(const char* envp[], size_t envc, void (*onCrash)(c
             JSC::Options::useJIT() = true;
             JSC::Options::useBBQJIT() = true;
             JSC::Options::useConcurrentJIT() = true;
+
+#if OS(LINUX) && CPU(X86_64)
+            // Workaround for JSC Wasm OSR bug on Linux x64 that causes crashes
+            // with repeated direct method calls on Emscripten WASM modules.
+            // https://github.com/oven-sh/bun/issues/26366
+            // https://bugs.webkit.org/show_bug.cgi?id=289009
+            JSC::Options::useWasmOSR() = false;
+#endif
             // JSC::Options::useSigillCrashAnalyzer() = true;
             JSC::Options::useSourceProviderCache() = true;
             // JSC::Options::useUnlinkedCodeBlockJettisoning() = false;
diff --git a/test/js/third_party/@electric-sql/pglite/pglite.test.ts b/test/js/third_party/@electric-sql/pglite/pglite.test.ts
index f26bb5ad17..61312790fc 100644
--- a/test/js/third_party/@electric-sql/pglite/pglite.test.ts
+++ b/test/js/third_party/@electric-sql/pglite/pglite.test.ts
@@ -1,11 +1,12 @@
 import { PGlite } from "@electric-sql/pglite";
-import { isCI, isLinux } from "harness";
 
 describe("pglite", () => {
-  // TODO(@190n) linux-x64 sometimes fails due to JavaScriptCore bug
-  // https://github.com/oven-sh/bun/issues/17841#issuecomment-2695792567
+  // This test previously failed on linux-x64 due to a JavaScriptCore Wasm OSR bug.
+  // Fixed by disabling useWasmOSR on linux-x64 in ZigGlobalObject.cpp.
+  // https://github.com/oven-sh/bun/issues/17841
+  // https://github.com/oven-sh/bun/issues/26366
   // https://bugs.webkit.org/show_bug.cgi?id=289009
-  it.todoIf(isCI && isLinux && process.arch == "x64")("can initialize successfully", async () => {
+  it("can initialize successfully", async () => {
     const db = new PGlite();
     expect(await db.query("SELECT version()")).toEqual({
       rows: [
diff --git a/test/regression/issue/26366.test.ts b/test/regression/issue/26366.test.ts
new file mode 100644
index 0000000000..2efbb4df9a
--- /dev/null
+++ b/test/regression/issue/26366.test.ts
@@ -0,0 +1,64 @@
+import { describe, expect, test } from "bun:test";
+import { bunEnv, bunExe } from "harness";
+
+// Regression test for https://github.com/oven-sh/bun/issues/26366
+// Bun crashes with SIGILL/segfault when calling an Emscripten-exported Wasm function
+// via direct method call on the module object after many iterations.
+// This is caused by a JSC Wasm OSR/JIT bug, fixed by disabling useWasmOSR on Linux x64.
+
+describe("issue #26366: JSC Wasm OSR crash with direct method calls", () => {
+  // The original bug only manifested on Linux x64, but this test validates
+  // the Wasm instantiation and direct method call pattern works on all platforms.
+  test("repeated wasm module instantiation with direct method calls", async () => {
+    // Create a minimal test that exercises the code path that would crash:
+    // - Multiple Wasm module instantiations
+    // - Direct method calls on the module object (module.export())
+    const script = `
+      // Minimal Wasm module: exports a function that returns its argument + 1
+      // Generated with: (module (func (export "add1") (param i32) (result i32) local.get 0 i32.const 1 i32.add))
+      const wasmBytes = new Uint8Array([
+        0x00, 0x61, 0x73, 0x6d, 0x01, 0x00, 0x00, 0x00, // magic + version
+        0x01, 0x06, 0x01, 0x60, 0x01, 0x7f, 0x01, 0x7f, // type section: (i32) -> i32
+        0x03, 0x02, 0x01, 0x00,                         // function section
+        0x07, 0x08, 0x01, 0x04, 0x61, 0x64, 0x64, 0x31, 0x00, 0x00, // export "add1"
+        0x0a, 0x09, 0x01, 0x07, 0x00, 0x20, 0x00, 0x41, 0x01, 0x6a, 0x0b, // code
+      ]);
+
+      // Use higher iterations on Linux x64 where the original crash occurred.
+      // Allow override via env var for CI stress testing.
+      const baseIterations = process.platform === 'linux' && process.arch === 'x64' ? 4000 : 500;
+      const iterations = parseInt(process.env.TEST_ITERATIONS, 10) || baseIterations;
+
+      async function runTest() {
+        for (let i = 0; i < iterations; i++) {
+          const wasmModule = await WebAssembly.compile(wasmBytes);
+          const instance = await WebAssembly.instantiate(wasmModule);
+
+          // Direct method call - this was the pattern that triggered the crash
+          const result = instance.exports.add1(i);
+
+          if (result !== i + 1) {
+            throw new Error(\`Unexpected result at iteration \${i}: expected \${i + 1}, got \${result}\`);
+          }
+        }
+        console.log(\`Completed \${iterations} iterations successfully\`);
+      }
+
+      await runTest();
+    `;
+
+    await using proc = Bun.spawn({
+      cmd: [bunExe(), "-e", script],
+      env: bunEnv,
+      stdout: "pipe",
+      stderr: "pipe",
+    });
+
+    const [stdout, stderr, exitCode] = await Promise.all([proc.stdout.text(), proc.stderr.text(), proc.exited]);
+
+    expect(stdout).toMatch(/Completed \d+ iterations successfully/);
+    expect(stderr).toBe("");
+    // The key assertion: process should exit normally without crash (SIGILL/SIGSEGV)
+    expect(exitCode).toBe(0);
+  });
+});